10月16日: Nucleic Acid Structure and Dynamics: Perspectives from Spin Labeling

2019-07-04 22:49:13 1

报告人:Peter Z. Qin 

(Department of Chemistry, University of Southern California, Los Angeles, California,)

 

报告时间:10月16日(星期二)下午13:00

 

  点:定量生物学中心(原理论生物学中心)老化学楼东配楼102会议室

       (理教路西/老光华楼北侧)


摘  要:

In site-directed spin labeling (SDSL), a stable nitroxide radical is covalently attached at a specific location within a macro-molecule, and structural and dynamic information at the labeling site is obtained via electron paramagnetic resonance (EPR) spectroscopy.[1] My group pioneered the development of a family of nitroxide probes, called the R5-series, which can be attached in an efficient and cost-effective manner to a specific phosphorothioate backbone position at arbitrary DNA or RNA sequences. Using these nucleotide-independent nitroxide probes in conjunction with pulse and continuous-wave EPR spectroscopy, we have obtained structural and dynamic information in systems that are difficult to study by other methods. In this presentation, I will describe recent work on mapping global structure of a non-coding RNA complex;[2, 3] probing nanosecond motions in a large RNA enzyme;[4] as well as investigating sequence-dependent DNA shape[5-7] and its role in specific recognition between the p53 tumor repressor and its DNA targets.  

主持人:欧阳颀教授