2018.12.27 Forward engineering of synthetic biological systems
题 目：Forward engineering of synthetic biological systems
报告人：Dr. Ye Chen（陈业）
Department of Biological Engineering, Massachusetts Institute of Technology
Synthetic biologists created genetic circuits for a variety of purposes, including scientific, biotechnological, industrial, and biomedical approaches. However, the construction processes are usually labor-intensive and unreliable. One critical challenge is the predictable tuning of the genetic circuit regulatory components within a gene circuit to elicit desired outputs. Here, we developed a method that allows one to tune the dynamic range in a motif-based construction of bacterial promoters with regulatory elements. Using this method, we constructed an orthogonal, tightly controlled two-signaling system. We then used this signaling system and two genetically distinct populations of Escherichia coli to engineer a bacterial consortium that exhibits robust oscillations in the gene expression. Furthermore, we extended and refined these engineering strategies to develop a toolbox for Saccharomyces cerevisiae (budding yeast) systems, with the aim to construct yeast promoters to integrate transcription signals. Using this toolbox, we applied the current computer-aided design platform, Cello, to the yeast gene circuit design. We achieved an 83% success rate in large circuit constructions (9–11 regulators, up to 20 regulatory operons).
Dr. Ye Chen received a B.S. in biotechnology at Zhejiang University in 2011 and a Ph.D. in biochemistry at Rice University in 2016. He is currently a postdoctoral researcher in Christopher Voigt’s laboratory at MIT. His research interest is synthetic biology, with a focus on 1) exploring the design principle in gene regulation, 2) developing genetic tools to control gene expressions, and 3) constructing systems based on model prediction.