2016.05.30 Mechanisms and Regulation of DNA Break Processing in Eukaryotes
题 目： Mechanisms and Regulation of DNA Break Processing in Eukaryotes
报告人： Niu Hengyao, Assistant Professor,
Department of Molecular and Cellular Biochemistry
Indiana University Bloomington
主持人：齐 志 研究员
Homologous recombination (HR) and non-homologous end joining (NHEJ) are conserved pathways for the repair of DNA double stranded breaks (DSBs) induced by ionizing radiation and genotoxic chemicals. HR utilizes a homologous template strand to repair damaged DNA in an error-free manner. NHEJ, which entails processing of the broken chromosome ends and their ligation, is often error-prone. HR is initiated by nucleolytic processing of the DNA break to yield 3' ssDNA tails for the recruitment of the repair machinery. Studies have revealed multiple pathways of DNA break resection. DNA break processing is regulated during the cell cycle. Specifically, NHEJ proteins suppress it in G1, but this suppression is relieved during S and G2. One focus of my research is to understand the mechanisms of the DNA end resection pathways and their regulation by NHEJ factors and chromatin assembly.
B.S. (1998) Peking University Cell Biology and Genetics
Ph.D. (2007) State University of New York, Stony Brook Molecular and Cellular Biology. Advisor: Nancy Hollingsworth.
Postdoctoral Training (2007-2014) Yale University School of Medicine. Mentor: Patrick Sung.
A long-term interest of their research is to delineate the mechanism of DSB (DNA double-strand break) repair and to ascertain its role in various DNA metabolic pathways. In particular, DSB repair takes place within complex organization of chromatin structures. Multiple chromatin modifications, remodelers and histone chaperons either participate directly in the repair process or function indirectly as regulators. Through a multi-disciplinary approach, They will strive to understand the interplay of DSB repair process and chromatin structures.