2015.7.22 NMR in Molecular Systems Biology: from structures to function
题目： NMR in Molecular Systems Biology: from structures to function
报告人： Prof. Lucia Banci
Professor of Chemistry at the University of Florence，Italy
Director and founder of the Center of Magnetic Resonance (CERM) of the University of Florence，Italy
NMR spectroscopy has unique potentialities in describing functional biological processes at atomic level and in a cellular context. It is indeed suitable not only to characterize the structure and dynamics of biomolecules but, even more importantly, to describe functional events still maintaining atomic resolution. This approach requires the development of suitable methodologies capable of addressing multiple, specific, and sometimes non conventional aspects and amenable to characterize functional processes in living cells, also integrating these data with those obtained in vitro.
Metal transfer processes occur through protein-protein interactions, with metal ion being transferred from one protein to the next, to the final recipient. The transfer is determined by metal affinity gradients among the various proteins, with kinetic contributing to the selectivity of the process. In cell NMR can provide the description of these processes within living cells.
The presence of paramagnetic centers, such as iron-sulfur clusters, dramatically affects the NMR spectra, requiring tailored experiments also integrated with EPR.
The power of NMR in describing cellular pathways at atomic resolution in a cellular environment will be presented for a few pathways responsible for copper trafficking in the cell and for the biogenesis of iron-sulfur proteins. New major advancements in in-cell NMR and in the characterization of highly paramagnetic systems will be also discussed within an integrated approach where, from single structures to protein complexes, the processes are described in their cellular context within a molecular perspective.
报告人简介：Lucia Banci，意大利佛罗伦萨大学化学系教授，欧洲最大的核磁中心CERM创始人之一，现任CERM中心主任，欧洲分子生物学组织（EMBO）和欧洲分子生物学实验室（EMBL）会员。其要研究方向为使用液体核磁共振的方法研究蛋白质的结构和功能，在金属蛋白结构，金属离子在细胞中的转移机制，in-cell NMR方法等方面作出了卓越的贡献。迄今在知名学术期刊如Nature，Nat Chem Biol, Nat Struct. Biol., PNAS, JACS等上发表高水平学术论文300多篇，解析了100多个蛋白质溶液结构，H-index指数62。在核磁共振领域享有很高的学术声望。