2014.3.31 YOUDONG (JACK) MAO
Single-Molecule Reconstruction of an HIV-1 Envelope Glycoprotein Precursor Trimer (gp160) by Cryo-Electron Microscopy
YOUDONG (JACK) MAO, PhD
Instructor in Microbiology and Immunobiology
Department of Cancer Immunology and AIDS, Dana-Farber CancerInstitute
Department of Microbiology and Immunobiology, Harvard MedicalSchool
Single-molecule reconstruction by cryo-electron microscopy (cryo-EM) is anemerging approach to analyze structure of protein complex in its native state.In this presentation, we will discuss the current state of the art of theapproach, its application to understand the molecular architecture of HIV-1membrane envelope glycoprotein trimeric precursor (gp160), as well as theprospect of further development of the approach and its potential impact onmolecular medicine in the near future. We will explain why the application ofsingle-molecule cryo-EM to the case of analysis of a metastable architecture ofHIV-1 gp160 trimer is unprecedented and likely leads to a revolution in modernstructural biology and molecular medicine. Comments on the current debate andcontroversy in the area of cryo-EM methodology development and application willbe given.