2015.6.4 CRISPR technology for genome regulation and applications
Title: CRISPR technology for genome regulation and applications
Speaker： Dr. Lei Stanley Qi
Assistant Professor, Departments of Bioengineering, Chemical and Systems Biology, and Stanford ChEM-H, Stanford University
Time： 10:00am June 4th 2015
Address： Rm 101, East wing of Old Chemistry Building, Peking Unversity
Chair： Dr. Ping Wei, Center for Quantitative Biology
Gene circuits allow programmable activation and repression of the genome. This provides a novel and powerful for both understanding biology and disease modeling. Here I will talk about our work on repurposing the bacterial CRISPR system as a synthetic biology toolset for sequence-specific gene regulation. The CRISPR tools enable multiplexable, inducible, and high-throughput activation or repression of genes across diverse organisms. The technology also allows genome-wide perturbation of gene expression for functional genomic research. We show several applications using the basic CRISPR tools, e.g., the construction of higher-order CRISPR circuits for modulating metabolic pathways in yeast. The CRISPR technology provides a powerful approach to engineering complex genetic systems for studying gene function and genomics.