2015.6.4 CRISPR technology for genome regulation and applications

2019-07-11 14:10:27 5

Title: CRISPR technology for genome regulation and applications

Speaker: Dr. Lei Stanley Qi

              Assistant Professor, Departments of Bioengineering, Chemical and Systems Biology, and Stanford ChEM-H, Stanford University

Time: 10:00am June 4th 2015


Address: Rm 101, East wing of Old Chemistry Building, Peking Unversity 

Chair:  Dr. Ping Wei,  Center for Quantitative Biology 



       Gene circuits allow programmable activation and repression of the genome. This provides a novel and powerful for both understanding biology and disease modeling. Here I will talk about our work on repurposing the bacterial CRISPR system as a synthetic biology toolset for sequence-specific gene regulation. The CRISPR tools enable multiplexable, inducible, and high-throughput activation or repression of genes across diverse organisms. The technology also allows genome-wide perturbation of gene expression for functional genomic research. We show several applications using the basic CRISPR tools, e.g., the construction of higher-order CRISPR circuits for modulating metabolic pathways in yeast. The CRISPR technology provides a powerful approach to engineering complex genetic systems for studying gene function and genomics.